Human PLA2G10 (Phospholipase A2, Group X) ELISA Kit - ELK4236

Human PLA2G10 (Phospholipase A2, Group X) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4236-48, ELK4236-96, ELK4236-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GXPLA2; GX sPLA2; sPLA2-X; Group 10 secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 10

Assay Type: Sandwich

Sensitivity: 3.5 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLA2G10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLA2G10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLA2G10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLA2G10 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O15496

Research Use Only