Human POMC (Proopiomelanocortin) ELISA Kit - ELK1875

Human POMC (Proopiomelanocortin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1875-48, ELK1875-96, ELK1875-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro Opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone

Assay Type: Competitive Inhibition

Sensitivity: 44 pg/mL

Standard: 10000 pg/mL

Detection Range: 156.25-10000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Endocrinology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human POMC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human POMC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human POMC in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P01189

Research Use Only