Human PP (Pepsin) ELISA Kit - ELK8433

Human PP (Pepsin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8433-48, ELK8433-96, ELK8433-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PP; Pepsin

Assay Type: Competitive Inhibition

Sensitivity: 0.93 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase; Tumor immunity; Gastroenterology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only