Product Description

Human PRKAb1 (Protein Kinase, AMP Activated Beta 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2246-48, ELK2246-96, ELK2246-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: AMPK, HAMPKb, 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit, Adenosine Monophosphate Activated Protein Kinase(AMPK)

Sensitivity: 0.125 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue Homogenates, Other Biological Fluids.

Assay Length: 3.5h

Research Area: Enzyme And Kinase, Metabolic Pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRKAb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRKAb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRKAb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRKAb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9Y478

Research Use Only