Product Description

Human PRKAb1 (Protein Kinase, AMP Activated Beta 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK2246-48, ELK2246-96, ELK2246-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRKAb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRKAb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRKAb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRKAb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: AMPK, HAMPKb, 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit, Adenosine Monophosphate Activated Protein Kinase(AMPK)

Reactivity: Human

Sample Type: Tissue Homogenates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Metabolic Pathway

Assay Length: 3.5h

Sensitivity: 0.125 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

UniProt: Q9Y478

Research Use Only