Product Description

Human PTPN5 (Tyrosine-Protein Phosphatase Non-Receptor Type 5) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9561-48, ELK9561-96, ELK9561-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: FLJ14427, Neural specific protein tyrosine phosphatase, Neural-specific protein-tyrosine phosphatase, Protein tyrosine phosphatase non receptor type 5 (striatum enriched), Protein tyrosine phosphatase non receptor type 5, Protein tyrosine phosphatase striatum enriched, PTN5, PTN5_HUMAN, PTP STEP, PTPN 5, Ptpn5, PTPSTEP, STEP, Striatum-enriched protein-tyrosine phosphatase, Tyrosine protein phosphatase non receptor type 5, Tyrosine-protein phosphatase non-receptor type 5,

Sensitivity: 0.052 ng/mL

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Neuro Science, Signal Transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTPN5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTPN5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTPN5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTPN5 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P54829

Research Use Only