Human PYY3 (Peptide YY 3) ELISA Kit - ELK6686

Human PYY3 (Peptide YY 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK6686-48, ELK6686-96, ELK6686-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PYY3; PYY-III

Assay Type: Competitive Inhibition

Sensitivity: 4.91 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PYY3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PYY3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PYY3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q5JQD4

Research Use Only