Human RESP18 (Regulated Endocrine Specific Protein 18) Microsample ELISA Kit - ELK7452MS

Human RESP18 (Regulated Endocrine Specific Protein 18) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7452MS-48, ELK7452MS-96, ELK7452MS-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Triskadecapeptide

Assay Type: Sandwich

Sensitivity: 29 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Endocrinology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RESP18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RESP18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RESP18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RESP18 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q5W5W9

Research Use Only