Human RPN1 (Ribophorin I) ELISA Kit - ELK4062

Human RPN1 (Ribophorin I) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4062-48, ELK4062-96, ELK4062-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: RPNI; OST1; RBPH1; Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase Subunit 1; Dolichyl-diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit

Assay Type: Sandwich

Sensitivity: 0.63 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RPN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RPN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RPN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RPN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P04843

Research Use Only