Human RPS6Ka1 (Ribosomal Protein S6 Kinase Alpha 1) ELISA Kit - ELK7155

Human RPS6Ka1 (Ribosomal Protein S6 Kinase Alpha 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7155-48, ELK7155-96, ELK7155-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HU-1; MAPKAPK1A; RSK; RSK1; S6K-alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; Ribosomal S6 kinase 1

Assay Type: Sandwich

Sensitivity: 0.122 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RPS6Ka1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RPS6Ka1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RPS6Ka1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RPS6Ka1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q15418

Research Use Only