Human S100A15 (S100 Calcium Binding Protein A15) ELISA Kit - ELK7320

Human S100A15 (S100 Calcium Binding Protein A15) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7320-48, ELK7320-96, ELK7320-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: S100A7A; S100A7L1; S100A7f; S100 calcium-binding protein A7-like 1

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100A15 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q86SG5

Research Use Only