Human SA (Sialic Acid) ELISA Kit - ELK8419

Human SA (Sialic Acid) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8419-48, ELK8419-96, ELK8419-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SA

Assay Type: Competitive Inhibition

Sensitivity: 3.83 µg/mL

Standard: 500 µg/mL

Detection Range: 7.82-500 µg/mL

Sample Type: Serum, plasma, other biological fluids

Assay Length: 2.5h

Research Areas: Signal transduction; Tumor immunity; Hepatology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only