Human SARS-CoV-2 Nucleocapsid ELISA Kit - ECV001

Human SARS-CoV-2 Nucleocapsid ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ECV001-48, ECV001-96, ECV001-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: COVID-19; SARS-CoV-2; 2019-nCoV

Assay Type: Sandwich

Sensitivity: 3.2 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Infectious Diseases

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only