Human SH3BGRL (SH3 Domain Binding Glutamic Acid Rich Protein Like Protein) ELISA Kit - ELK7085

Human SH3BGRL (SH3 Domain Binding Glutamic Acid Rich Protein Like Protein) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7085-48, ELK7085-96, ELK7085-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SH3BGR; SH3BGRL

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SH3BGRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SH3BGRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SH3BGRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SH3BGRL in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O75368

Research Use Only