Human sHLA-G (soluble human leucocyte antigen G) ELISA Kit - ELK9364

Human sHLA-G (soluble human leucocyte antigen G) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9364-48, ELK9364-96, ELK9364-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: sHLA-G

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Apoptosis; Tumor immunity; Infection immunity; Immune molecule; Hematology; Reproductive science; Immunodeficiency; Autoimmunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sHLA-G. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sHLA-G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sHLA-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sHLA-G in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only