Human sIgA (Secretory Immunoglobulin A) ELISA Kit - ELK1844

Human sIgA (Secretory Immunoglobulin A) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1844-48, ELK1844-96, ELK1844-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: S-IgA

Assay Type: Sandwich

Sensitivity: 0.099 µg/mL

Standard: 20 µg/mL

Detection Range: 0.32-20 µg/mL

Sample Type: serum, plasma, saliva, stool, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Infection immunity; Immune molecule; Reproductive science; Genetic science; Dermatology; Immunodeficiency;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only