Human SLC1A5 (Solute Carrier Family 1, Member 5) ELISA Kit - ELK3497

Human SLC1A5 (Solute Carrier Family 1, Member 5) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK3497-48, ELK3497-96, ELK3497-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: SLC1A5; AAAT; ASCT2; ATBO; M7V1; M7VS1; R16; RDRC; Neutral Amino Acid Transporter B (0); Baboon M7 virus receptor; Sodium-dependent neutral amino acid transporter type 2

Assay Type: Sandwich

Sensitivity: 0.121 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLC1A5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC1A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLC1A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLC1A5 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q15758

Research Use Only