Human SLC5A8 (Solute Carrier Family 5, Member 8) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10077-48, ELK10077-96, ELK10077-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Sodium-coupled monocarboxylate transporter 1; SLC5A8; Solute carrier family 5 member 8; AIT; SMCT ; SMCT1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter
Assay Type: Sandwich
Sensitivity: 0.094 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Areas: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLC5A8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC5A8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLC5A8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLC5A8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8N695
Research Use Only