Human Tau (Tau Proteins) ELISA Kit - ELK11225

Human Tau (Tau Proteins) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11225-48, ELK11225-96, ELK11225-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Mapt ; Mtapt; Tau; Microtubule-associated protein tau; Microtubule-associated protein tau; Paired helical filament-tau (PHF-tau)

Assay Type: Sandwich

Sensitivity: 5.4 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Signal Transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Tau. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tau. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Tau, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tau in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P10636

Research Use Only