Human Tb4 (Thymosin Beta 4) ELISA Kit - ELK4391

Human Tb4 (Thymosin Beta 4) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4391-48, ELK4391-96, ELK4391-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide

Assay Type: Competitive Inhibition

Sensitivity: 0.88 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Endocrinology; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Tb4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Tb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Tb4 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P62328

Research Use Only