Human TC (Total cholesterol) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8420-48, ELK8420-96, ELK8420-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: TC
Assay Type: Competitive Inhibition
Sensitivity: 2.76 µg/mL
Standard: 500 µg/mL
Detection Range: 7.82-500 µg/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Hepatology; Gastroenterology; Nutrition metabolism; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TC in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only