Product Description

Human TIEG1 (TGF Beta Inducible Early Response Gene 1) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1827MS-48, ELK1827MS-96, ELK1827MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIEG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TIEG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TIEG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIEG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternate Names: KLF10, EGRA, TIEG, Krueppel-Like Factor 10, TGFB Inducible Early Growth Response, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Apoptosis, Tumor Immunity, Infection Immunity, Hepatology

Assay Length: 3.5h

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Method: 0.32-20 ng/mL

UniProt: Q13118

Research Use Only