Human TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit - ELK9590

Human TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9590-48, ELK9590-96, ELK9590-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: ACP5; TRAP5b; SPENCDI

Assay Type: Sandwich

Sensitivity: 0.45 ng/mL

Standard: 50 ng/mL

Detection Range: 0.78-50 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal Transduction; Signal Transduction; Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only