Human TRH (Thyrotropin Releasing Hormone) ELISA Kit - ELK2641

Human TRH (Thyrotropin Releasing Hormone) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK2641-48, ELK2641-96, ELK2641-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor

Assay Type: Competitive Inhibition

Sensitivity: 2.7 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Endocrinology; Neuroscience; Hepatology; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRH in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P20396

Research Use Only