Human TSLP (Thymic Stromal Lymphopoietin) Microsample ELISA Kit - ELK1083MS

Human TSLP (Thymic Stromal Lymphopoietin) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1083MS-48, ELK1083MS-96, ELK1083MS-5x96

Citations, Manuals and MSDS Available upon request.

Reactivity: Human

Alternative Names: Pro-MMP-9

Assay Type: Sandwich

Sensitivity: 0.478 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase; Tumor immunity; Infection immunity; Cardiovascular biology; Hepatology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-MMP-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Pro-MMP-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Pro-MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Pro-MMP-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only