Human UBD (Ubiquitin D) ELISA Kit Sizes: 48T, 96T 96T*5 Catalogue Number: ELK2165-48, ELK2165-96, ELK2165-96*5 Lead times: 1-2 weeks, if manufacturer has product in stock Manufacturer/Ship Location: China Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UBD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UBD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UBD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UBD in the samples is then determined by comparing the OD of the samples to the standard curve. Alternative Names: UB-D, FAT10, UBD-3, Diubiquitin, Ubiquitin-like protein FAT10 Reactivity: Human Sample Type: Serum, Plasma, Other Biological Fluids  Assay Type: Sandwich Research Areas: Infection Immunity, Developmental Science Assay Length: 3.5h Sensitivity: 1.4 ng/mL Standard: 200 ng/mL Detection Range: 3.13-200 ng/mL UniProt: O15205 Research Use Only