Human VD (Vitamin D) ELISA Kit - ELK11264

Human VD (Vitamin D) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11264-48, ELK11264-96, ELK11264-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: VD; VD1; Lamisterol; Vitamin D; Vitamin D1; Lumisterol

Assay Type: Competitive Inhibition

Sensitivity: 2.19 ng/mL

Standard: 400 ng/mL

Detection Range: 6.25-400 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Metabonomics

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only