Human WNT16 (Wingless Type MMTV Integration Site Family, Member 16) ELISA Kit - ELK11383

Human WNT16 (Wingless Type MMTV Integration Site Family, Member 16) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11383-48, ELK11383-96, ELK11383-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: Protein Wnt 16 precursor; Protein Wnt-16; WNT16;

Assay Type: Sandwich

Sensitivity: 27.6 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WNT16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WNT16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WNT16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WNT16 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9UBV4

Research Use Only