Human WWOX (WW domain-containing oxidoreductase) ELISA Kit - ELK9495

Human WWOX (WW domain-containing oxidoreductase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9495-48, ELK9495-96, ELK9495-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: WWOX; FOR; WOX1; WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase

Assay Type: Sandwich

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 3.5h

Research Areas: Cancer

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WWOX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WWOX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WWOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WWOX in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9NZC7

Research Use Only