HUS1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A5407-20, A5407-100
Citations, Manuals and MSDS Available upon request.
Background: The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse
Protein Weight: 32kDa
Observed Molecular Weight: 35kDa
Immunogen: Recombinant protein (or fragment) .
Species: Human
GeneID: 3364
SWISS: O60921
Alternate Names: hHUS1; HUS1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:100 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, DNA Damage Repair, Signal Transduction, Kinase.
NCBI Alias: HUS1
Research Use Only