HVD3 (25-Hydroxyvitamin D3) ELISA Kit - ELK7890

HVD3 (25-Hydroxyvitamin D3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7890-48, ELK7890-96, ELK7890-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25 (OH)D

Assay Type: Competitive Inhibition

Sensitivity: 0.91 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Length: 2h

Research Areas: Reproductive science; Genetic science; Nutrition metabolism; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only