Product Description

HVD3 (25-Hydroxyvitamin D3) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK7890-48, ELK7890-96, ELK7890-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: 25-HVD3, 25HVD3, Calcifediol, Calcidiol, H-VD3, 25-Hydroxycholecalciferol, 25-Hydroxy Vitamin D3, 25(OH)D

Reactivity: General

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Reproductive Science, Genetic Science, Nutrition Metabolism, Bone Metabolism

Assay Length: 2h

Sensitivity: 0.91 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Research Use Only