Hyp (Hydroxyproline) ELISA Kit - ELK7835

Hyp (Hydroxyproline) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7835-48, ELK7835-96, ELK7835-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: (2S,4R)-4-Hydroxyproline; L-Hydroxyproline (C5H9O3N)

Assay Type: Competitive Inhibition

Sensitivity: 25.4 ng/mL

Standard: 5000 ng/mL

Detection Range: 78.13-5000 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Nutrition metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hyp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only