IAA (Indole 3 Acetic Acid) ELISA Kit - ELK7852

IAA (Indole 3 Acetic Acid) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK7852-48, ELK7852-96, ELK7852-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin

Assay Type: Competitive Inhibition

Sensitivity: 0.92 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: tissue or cell culture supernates

Assay Length: 2h

Research Areas: Signal transduction; Hormone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IAA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IAA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IAA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only