Product Description

IFN-α Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60294-50, BS60294-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P01562

Host: Rabbit

Reactivity: Human

Applications: WB,

All Applications: WB: 1:1000~1:2000

Background: Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection. They also affect cell differentiation, growth, surface antigen expression, and immunoregulation. There are three naturally occurring interferons: α, β, and γ. IFN-α is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. Recombinant IFN-α from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines. When binding to discrete cell surface receptors on target cells, IFN-α induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway. IFN-α signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (β and ε). All IFN-α signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IFN-α polyclonal antibody detects endogenous levels of IFN-α protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IFN-α polyclonal antibody at 1:1000 dilution
Lane 1: IFN-α recombinant protein(0.2ug)

Alternative Name: Interferon alpha-1/13; IFN-alpha-1/13; Interferon alpha-D (LeIF D); IFNA1; IFNA13

Immunogen: Synthetic peptide, corresponding to Human IFN-α.

Conjugate: Unconjugated

Modification: Unmodified