Product Description

IGF-IR (phospho Tyr1165/Y1166) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1335-50, ES1335-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014],

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Applications: WB, ELISA

Alternate Names: IGF1R, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD antigen CD221

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human IGF1R around the phosphorylation site of Tyr1165/Tyr1166. AA range:1131-1180

Isotype: IgG

Target: IGF-1R

Specificity: Phospho-IGF-IR (Y1165/Y1166) Polyclonal Antibody detects endogenous levels of IGF-IR protein only when phosphorylated at Y1165/Y1166.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level), Expressed in a variety of tissues, Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney,

Observed Band (KD): pro: 155kD, recetor beta: 95kD

Fields: EGFR tyrosine kinase inhibitor resistance, Endocrine resistance, MAPK signaling pathway, Ras signaling pathway, Rap1 signaling pathway, HIF-1 signaling pathway, FoxO signaling pathway, Oocyte meiosis, Autophagy - animal, Endocytosis, mTOR signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Longevity regulating pathway, Longevity regulating pathway - multiple species, Focal adhesion, Adherens junction, Signaling pathways regulating pluripotency of stem cells, Long-term depression, Ovarian steroidogenesis, Progesterone-mediated oocyte maturation, Pathways in cancer, Transcriptional misregulation in cancer, Proteoglycans in cancer, Glioma, Prostate cancer, Melanoma, Breast cancer, Hepatocellular carcinoma

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Disease: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM: 270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1
Enzyme Regulation: Autophosphorylation activates the kinase activity
Function: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed: 12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low a

Subcellular Location: Cell membrane, Single-pass type I membrane protein

Storage and Stability: -20°C/1 year

Gene Name: IGF1R

Protein Name: Insulin-like growth factor 1 receptor

Mouse Gene ID: 16001

Mouse SwissProt Number: Q60751

Rat Gene ID: 25718

Rat SwissProt Number: P24062

Research Use Only