Product Description

Instant ELISA Kit for Indole 3 Acetic Acid (IAA)

Sizes: 24T, 48T, 96T

Catalogue Numbers: IEA737Ge-24, IEA737Ge-48, IEA737Ge-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Featured Series Function: Detects protein (quick procedure in 1 hrs)

Method: Colormetric

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Instant Indole 3 Acetic Acid (IAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Instant Indole 3 Acetic Acid (IAA) and unlabeled Instant Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Instant Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Instant Indole 3 Acetic Acid (IAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Instant Indole 3 Acetic Acid (IAA) in the sample.

Reactivity: General

Alternate Names: IA-A, Indolylacetic Acid, Indoleacetic Acid, Heteroauxin

Target: Indole 3 Acetic Acid,

Assay Length: 70min

Sample Size: 50uL

Sample Type: Tissue or Cell Culture Supernates

Detection Method: Competitive Inhibition

Detection Range: 320-200,000pg/mL

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Indole 3 Acetic Acid (IAA) were tested 20 times on one plate, respectively.Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.CV(%) = SD/meanX100

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Assay Procedure: 1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample to each well.And then add 50µL prepared Detection Reagent A immediately.Share and mix. Incubate 50 minutes at 37°C; 3. Aspirate and wash 3 times; 4. Add 100µL prepared Detection Reagent B. Incubate 10 minutes at 37°C; 5. Aspirate and wash 5 times; 6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450nm immediately.

Specificity: Reactive with General species IAA / Indole 3 Acetic Acid

Sensitivity: 109pg/mL

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Shelf Life: 12 months

Research Use Only