Product Description

IRF3 (Phospho-S385) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4609 -50, BS4609 -100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Interferon regμlatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regμlators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particμlarly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regμlating cell growth, overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP).

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC, IF/ICC

Alternate Names: Interferon regμlatory factor 3, IRF-3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S385 of human IRF3 protein.

Dilution: WB: 1:500~1:1000
IHC: 1:100~1:200
IF/ICC: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IRF3 (Phospho-S385) polyclonal antibody detects endogenous levels of IRF3 protein only when phosphorylated at Ser385.

Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q14653

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only