Product Description

IRS-1 (phospho Ser1101) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1339-50, ES1339-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: IRS1, Insulin receptor substrate 1, IRS-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser1101. AA range:1067-1116

Isotype: IgG

Target: IRS-1

Specificity: Phospho-IRS-1 (S1101) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S1101.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Epithelium, Eye, Skeletal muscle,

Observed Band (KD): 170kD

Fields: cGMP-PKG signaling pathway, FoxO signaling pathway, Autophagy - animal, mTOR signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Longevity regulating pathway, Longevity regulating pathway - multiple species, Neurotrophin signaling pathway, Insulin signaling pathway, Adipocytokine signaling pathway, Regulation of lipolysis in adipocytes, Type II diabetes mellitus, Insulin resistance, Non-alcoholic fatty liver disease, Growth hormone synthesis, secretion and action, Aldosterone-regulated sodium reabsorption, Alzheimer disease, MicroRNAs in cancer, Diabetic cardiomyopathy

Function: Disease: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM: 125853]
Function: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
polymorphism: The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependen

Subcellular Location: nucleus, cytoplasm, cytosol, plasma membrane, insulin receptor complex, caveola, intracellular membrane-bounded organelle,

Storage and Stability: -20°C/1 year

Gene Name: IRS1

Protein Name: Insulin receptor substrate 1

Mouse Gene ID: 16367

Mouse SwissProt Number: P35569

Rat Gene ID: 25467

Rat SwissProt Number: P35570

Research Use Only