[KO Validated] CDK4 Rabbit PolymAb® - A23521PM

[KO Validated] CDK4 Rabbit PolymAb®

Sizes: 20μL, 100μL

Catalogue Numbers: A23521PM-20, A23521PM-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 (INK4a) . This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb) . Mutations in this gene as well as in its related proteins including D-type cyclins, p16 (INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Category: Monoclonal Antibodies

Applications: WB, IP, IF/ICC, IF-P, IHC-P, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 34kDa

Observed Molecular Weight: 34kDa

Immunogen: Synthetic peptide .

Species: Human

GeneID: 1019

SWISS: P11802

Alternate Names: CMM3; PSK-J3

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Recommended Dilutions: WB, 1:1000 - 1:12000 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells IF/ICC, 1:200 - 1:800 IF-P, 1:200 - 1:800 IHC-P, 1:200 - 1:800 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Epigenetics Nuclear Signaling, Protein phosphorylation, Cancer, Signal Transduction, Kinase, Serine threonine kinases, Cell Biology Developmental Biology, Cell Cycle, Cell Cycle Control-G1 S Checkpoint.

NCBI Alias: CDK4

Research Use Only