[KO Validated] CDKN1A/p21 Rabbit PolymAb® - A22460PM

[KO Validated] CDKN1A/p21 Rabbit PolymAb®

Sizes: 20μL, 100μL

Catalogue Numbers: A22460PM-20, A22460PM-100

Citations, Manuals and MSDS Available upon request.

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Category: Monoclonal Antibodies

Applications: WB, IF/ICC, ELISA

Cross-reactivity: Human, Mouse

Protein Weight: 18 kDa

Observed Molecular Weight: 21 kDa

Immunogen: Recombinant protein (or fragment) .

Species: Human/Mouse

GeneID: 1026/12575

SWISS: P38936/P39689

Alternate Names: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Recommended Dilutions: WB, 1:1000 - 1:10000 IF/ICC, 1:1000 - 1:4000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1;.

NCBI Alias: CDKN1A/p21

Research Use Only