[KO Validated] IDH1 Rabbit pAb - A13245

[KO Validated] IDH1 Rabbit pAb

Sizes: 20μL, 100μL

Catalogue Numbers: A13245-20, A13245-100

Citations, Manuals and MSDS Available upon request.

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+) -dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+) -dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+) -dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+) -dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Category: Polyclonal Antibodies

Applications: WB, IF/ICC, IP, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 47kDa

Observed Molecular Weight: 46kDa

Immunogen: Recombinant protein (or fragment)

Species: Human

GeneID: 3417

SWISS: O75874

Alternate Names: IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26; H1

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH 7.3.

Recommended Dilutions: WB, 1:500 - 1:1000 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Epigenetics Nuclear Signaling, Cancer, Signal Transduction, Endocrine Metabolism, Lipid Metabolism.

NCBI Alias: IDH1

Research Use Only