[KO Validated] Laminin beta 1 Rabbit pAb - A19970

[KO Validated] Laminin beta 1 Rabbit pAb

Sizes: 20μL, 100μL

Catalogue Numbers: A19970-20, A19970-100

Citations, Manuals and MSDS Available upon request.

Background: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

Category: Polyclonal Antibodies

Applications: WB, ELISA

Cross-reactivity: Human, Mouse

Protein Weight: 198kDa

Observed Molecular Weight: 230kDa

Immunogen: Recombinant protein (or fragment) .

Species: Human

GeneID: 3912

SWISS: P07942

Alternate Names: CLM; LIS5; 1

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3.

Recommended Dilutions: WB, 1:1000 - 1:4000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Signal Transduction, Cell Biology Developmental Biology, Cytoskeleton, Extracellular Matrix, Cardiovascular, Angiogenesis.

NCBI Alias: LAMB1

Research Use Only