[KO Validated] PRKAR1A Rabbit pAb - A0906

[KO Validated] PRKAR1A Rabbit pAb

Sizes: 20μL, 100μL

Catalogue Numbers: A0906-20, A0906-100

Citations, Manuals and MSDS Available upon request.

Background: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.

Category: Polyclonal Antibodies

Applications: WB, IF/ICC, ELISA

Cross-reactivity: Human, Mouse, Rat

Protein Weight: 43kDa

Observed Molecular Weight: 48kDa

Immunogen: Recombinant protein (or fragment)

Species: Human

GeneID: 5573

SWISS: P10644

Alternate Names: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1; 1A

Source: Rabbit

Isotype: IgG

Purity: Affinity purification

Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.

Recommended Dilutions: WB, 1:500 - 1:1000 IF/ICC, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Research Areas: Cancer, Signal Transduction, G protein signaling, G-Protein-Coupled Receptors Signaling to MAPK Erk, Kinase, Serine threonine kinases, MAPK-Erk Signaling Pathway, Cell Biology Developmental Biology, Apoptosis, Mitochondrial Control of Apoptosis, Inhibition of Apoptosis, Cytoskeleton, Microtubules, Actins, Endocrine Metabolism, AMPK Signaling Pathway, Insulin Receptor Signaling Pathway, Immunology Inflammation, Neuroscience, Neurodegenerative Diseases, Dopamine Signaling in Parkinson's Disease.

NCBI Alias: PRKAR1A

Research Use Only