(KO Validated) SMAD2 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-62120-60, E-AB-62120-120, E-AB-62120-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: SMAD2
Target Synonym: JV18; JV18-1; MADH2; MADR2; hMAD-2; hSMAD2; Smad2; SMAD2
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q15796
Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
Concentration: 1mg/mL
Immunogen: A synthetic peptide of human SMAD2 (NP_001003652.1).
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Calculated MW: 48kDa/52kDa
Observed MW: 60kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only