Product Description

Ku-80 (phospho Thr714) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES7520-50, ES7520-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],

Reactivity: Human, Monkey

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: XRCC5, G22P2, X-ray repair cross-complementing protein 5, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair pr

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Ku80 around the phosphorylation site of Thr714. AA range:683-732

Isotype: IgG

Target: Ku-80

Specificity: Phospho-Ku-80 (T714) Polyclonal Antibody detects endogenous levels of Ku-80 protein only when phosphorylated at T714.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Cervix carcinoma, Coronary artery, Heart, Neuroblastoma, Osteoblast, Thy

Observed Band (KD): 83kD

Fields: Non-homologous end-joining

Function: Developmental Stage: Expression increases during promyelocyte differentiation
Disease: Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86
Domain: The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage
Function: Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of t

Subcellular Location: Nucleus Nucleus, nucleolus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: XRCC5

Protein Name: X-ray repair cross-complementing protein 5

Mouse SwissProt Number: P27641

Research Use Only