Product Description

Kv2.1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5993-50, ES5993-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shab-related subfamily. This member is a delayed rectifier potassium channel and its activity is modulated by some other family members. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC, IF, ELISA

Alternate Names: KCNB1, Potassium voltage-gated channel subfamily B member 1, Delayed rectifier potassium channel 1, DRK1, h-DRK1, Voltage-gated potassium channel subunit Kv2.1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Kv2.1/KCNB1. AA range:533-582

Isotype: IgG

Target: Kv2.1

Specificity: Kv2.1 Polyclonal Antibody detects endogenous levels of Kv2.1 protein.

Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in neocortical pyramidal cells (PubMed:24477962), Expressed in pancreatic beta cells (at protein level) (PubMed:12403834, PubMed:14988243), Expressed in brain, heart, lung, liver, colon, kidney and adrenal gland (PubMed:19074135), Expressed in the cortex, amygdala, cerebellum, pons, thalamus, hypothalamus, hippocampus and substantia nigra (PubMed:19074135),

Molecular Weight (Da): 96kD

Function: Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position
Domain: The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments
Function: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient
PTM: Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-656 and Ser-720 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-

Subcellular Location: Cell membrane Perikaryon Cell projection, axon Cell projection, dendrite Membrane, Multi-pass membrane protein Cell junction, synapse, postsynaptic cell membrane Cell junction, synapse Cell junction, synapse, synaptosome Lateral cell membrane Cell membrane, sarcolemma Localizes to high-density somatodendritic clusters and non-clustered sites on the surface of neocortical and hippocampal pyramidal neurons in a cortical actin cytoskeleton-dependent manner (PubMed:24477962) Localizes also to high-density clusters in the axon initial segment (AIS), at ankyrin-G-deficient sites, on the surface of neocortical and hippocampal pyramidal neurons (PubMed:24477962) KCNB1-containing AIS clusters localize either in close apposition to smooth endoplasmic reticulum cisternal organell

Storage and Stability: -20°C/1 year

Gene Name: KCNB1

Protein Name: Potassium voltage-gated channel subfamily B member 1

Mouse Gene ID: 16500

Mouse SwissProt Number: Q03717

Rat Gene ID: 25736

Rat SwissProt Number: P15387

Research Use Only