L-Lactic Acid (LA) Colorimetric Assay Kit - E-BC-K044-M

L-Lactic Acid (LA) Colorimetric Assay Kit

Sizes: 48T, 96T, 500 Assays

Catalogue Numbers: E-BC-K044-M-48, E-BC-K044-M-96, E-BC-K044-M-500

Citations, Manuals and MSDS Available upon request.

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: Serum, plasma, cell culture supernatant, tissue, cells

Assay Time: 30 min

Detection Type: Colorimetric method

Sensitivity: 0.10 mmol/L

Detection Range: 0.12-7.0 mmol/L

Test Principle: Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.

Inter CV: 3.5

Intra CV: 1.4

Storage: This product can be stored at 2~8°C for 12 months with shading light.

Shipping: Ice bag