LA (Lactic Acid) ELISA Kit - ELK8217

LA (Lactic Acid) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8217-48, ELK8217-96, ELK8217-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: LA

Assay Type: Competitive Inhibition

Sensitivity: 2.96 µg/mL

Standard: 600 µg/mL

Detection Range: 9.38-600 µg/mL

Sample Type: serum, plasma and biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase; Cardiovascular biology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to LA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LA in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only