Lactate Dehydrogenase (LDH) Activity Assay Kit
Sizes: 96T, 500 Assays
Catalogue Numbers: E-BC-K046-M-96, E-BC-K046-M-500
Citations, Manuals and MSDS Available upon request.
Target Synonym: LDH
Research Areas: Liver and Renal Function; Glycolysis and Lipid Metabolism
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, hydrothorax, tissue, cells
Assay Time: 45 min
Detection Type: Colorimetric method
Sensitivity: 6 U/L
Detection Range: 6-1000 U/L
Test Principle: Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value.
Inter CV: 2.4
Intra CV: 1.8
Storage: This product can be stored at 2~8°C for 12 months with shading light.
Shipping: Ice bag