Product Description

Lfc Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2709-50, ES2709-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA, IHC

Alternate Names: ARHGEF2, KIAA0651, LFP40, Rho guanine nucleotide exchange factor 2, Guanine nucleotide exchange factor H1, GEF-H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from C-ternal human ARHGEF2. AA range:851-900

Isotype: IgG

Target: Lfc

Specificity: Lfc Polyclonal Antibody detects endogenous levels of Lfc protein.

Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 2000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Cervix carcinoma, Epithelium, Platelet,

Observed Band (KD): 111kD

Fields: Tight junction, Pathogenic Escherichia coli infection, Shigellosis, Fluid shear stress and atherosclerosis

Function: Domain: The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA
Domain: The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions
Function: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed: 9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases
Online Information: ARHGEF2 entry,PTM: Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activit

Subcellular Location: Cytoplasm, cytoskeleton Cytoplasm Cell junction, tight junction Golgi apparatus Cytoplasm, cytoskeleton, spindle Cell projection, ruffle membrane Cytoplasmic vesicle Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization (PubMed:15827085) Recruited into membrane ruffles induced by Sflexneri at tight junctions of polarized epithelial cells (PubMed:19043560) Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730)

Storage and Stability: -20°C/1 year

Gene Name: ARHGEF2

Protein Name: Rho guanine nucleotide exchange factor 2

Mouse Gene ID: 16800

Mouse SwissProt Number: Q60875

Rat Gene ID: 310635

Rat SwissProt Number: Q5FVC2

Research Use Only