Product Description

Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents)

Sizes: 50Assays, 100Assays

Catalogue Numbers: E-BC-K205-S-50, E-BC-K205-S-100

Lead times: 2-3 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: Lipoproteins (except low-density lipoprotein cholesterol (LDL)) such as high density lipoprotein (HDL), chylomicron (CM), and very low density lipoprotein (VLDL) change structure and dissociate under the action of surfactants. The released micronized cholesterol molecules react with cholesterol enzyme reagents, and the generated hydrogen peroxide is trapped in the absence of coupling agent. It is consumed without color development. At this time, the LDL particles are still intact, and then the reagent containing coupling agent is added, which can dissociate the LDL particles to release cholesterol, which is catalyzed by cholesterol esterase (CE) and cholesterol oxidase (CO) and produce hydrogen peroxide. Hydrogen peroxide is catalyzed by oxidase (POD) in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound, the shade of color is directly proportional to the LDL-C content.

Abbreviation: LDL-C

Research Areas: Metabolic Diseases

Result Type: Quantitative

Detection Instrument: Spectrophotometer

Detection Type: Colorimetric method

Detection Range: 0.061-12 mmol/L

Tested Sample Types: Serum;Plasma;animal tissue

Assay Length: 50 min

Sensitivity: 0.061 mmol/L

Inter cv: 3.2-7.3%

Intra cv: 1.1-4.8%

Research Use Only